최근연구논문

Methionine Adenosyltransferase 1: 간경화 환자에서 단백체학 분석을 통한 조기 간세포암 진단표지자

논문 작성자
이주호, 전미정, 심주현, 송기원, 탁은영, 오보라, 유은실, 최상운, 안지현, 이단비, 김강모, 임영석, 이한주, 정영화, 이영상
논문 게재지
Journal of liver cancer
논문 게재년
2018
논문 게재월
03
이미지

Methionine Adenosyltransferase 1: 간경화 환자에서 단백체학 분석을 통한 조기 간세포암 진단표지자


 


이주호, 전미정, 심주현, 송기원, 탁은영, 오보라, 유은실, 최상운, 안지현, 이단비, 김강모, 임영석, 이한주, 정영화, 이영상


Journal of liver cancer, 18(1):33-43, doi:10.17998/jlc.18.1.33


ABSTRACT


Background: Because there is a lack of effective biomarkers, we aimed to discover proteomic candidate markers for hepatocellular carcinoma (HCC) in cirrhotic patients at the highest-risk of HCC, and to validate the markers.


Materials and Methods: We collected tumor tissue from 5 cirrhotics with HCC, and from 5 cirrhotics without HCC, who underwent liver resection or transplantation. These tissue samples were analyzed by 2-dimensional difference gel electrophoresis coupled with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and potential markers were validated at the transcriptional and translational levels. We also performed western blot assays using other blood samples from 10 cirrhotics with HCC and 10 without HCC.


Results: Among the 66 distinguishable spots on 2-D gel images, we identified 15 proteins overexpressed more than 1.5 fold in terms of volume ratio in the tumors. Ten of the over-expressed proteins were identified by MALDI-TOF MS; of those, only methionine adenosyltransferase 1 (MAT1), a protein specific for liver, and acyl-CoA dehydrogenase were significantly up-regulated in tumors in further immunoblotting analyses (Ps<0.05). There was no between-pair difference in MAT1 mRNA measured by real-time polymerase chain reaction (P=0.96). However, in western blots of serum samples, distinct MAT1 bands were observed in all 10 HCC patients, but in only 2 of the non-HCC patients.


Conclusions: MAT1 is a potential marker for surveillance in cirrhotic patients with and without prior HCC


- Fulltext: http://kiss.kstudy.com/thesis/thesis-view.asp?key=3584063